Disconnect the column and attach a union and flow directly to waste.
Waters hplc system cleaning procedure.
Reverse osmosis to remove most contaminants b.
Label bottles correctly with bottle content and filling date expiry date.
Water flush to clean acid from the system.
Residues or contaminations may block filters or capillaries.
Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307.
3 0 responsibility 3 1 doing.
This sop shall be applicable for the hplc system waters alliances 2690 2695 separation modules.
To clean the system.
Technical assistant 3 2 checking.
When preparing a system to run your method it is always important to prime up the system to ensure you get fresh solvents in all of the required lines.
Cleaning decontamination columns general guidelines flow cells general.
The purification process should include all of the following steps.
Responsibility quality control officer executive.
Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
To clean the system.
Ion exchange to remove any remaining ions.
The water flushes are only needed to remove the previous solvent from the system.
When working with any lc system setting it up and storing it is very important.
Use only hplc grade solvents and water filtered through 0 2.
For information on other acquity uplc cleaning methods refer to the recommended cleaning mixtures for lc table in the con trolling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
Water acetonitrile methanol ipa 1 1 1 1 with 2 5 fa.
It is necessary to restore the system flow path to a near new level of cleanliness note.
10 nh4oh in water this removes peg and other.
Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems.
Remove all solvent filters.
Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data.
Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning.
Controlling contamination in lc ms and hplc ms systems 715001307.
Maintaining your hplc uhplc uplc systems.
Water flush to clear customer s mobile phases from the system.
Use solvent inlet filters to protect the system from incoming particles.
No injections are needed with that.
